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KMID : 0350519940470041691
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Journal of Catholic Medical College
1994 Volume.47 No. 4 p.1691 ~ p.1703
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Effect of and Extracellular Fluid Type Solution on Lung Preservation of an Isolated Rabbit Heart-Lung Block Model
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Abstract
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Lung transplantation has been employed successfully for the treatment of end stage pulmonary vascular and parenchymal diseases. Limited donor supply is the major factor restricting the application of lung transplantation. A uniformly reliable
method of
lung preservation would improve donor organ availability. Recently, Euro-Collins'(Euro-C) solution, an intracellular fluid type solution. is most widely used in organ preservation.
In the present study, we used an in vitro-ventilated, blood perfused rabbit lung model to examine the effects of extracellular fluid type solution(Low-Potassium-Dextran-Glucose; IPD) and modified Euro-Collins' colution(Euro-C) during pulmonary
flush and
preservation period.
For this study, animals were divided into three groups: control group(n=6), nonpreserved lungs; LPD group(n=24), lungs were flushed with LPD) solution and stored(10℃) in the same flushy solution for 6 hours(n=6), 12 hours(n=6), 18 hours(n=6),
and
24
hours(n=6); Euro-C group(n=24), lungs flushed and stored with modified Euro-collins' solution for same period of LPD group.
Rabbit lungs were harvested and stored after pulmonary flush with same solutions. The lungs were preserved at 10℃. On completion of each storage period(6, 12, 18 and 24 hours), the lung was ventilated and reperfused with rabbit venous blood at a
rate
of 30ml/min for 10 minutes. Assess-ment of lung functions included pulmonary vascular resistance during pulmonary flush, gas analysis of effluent blood, pulmonary artery pressure during reperfusion and wet/dry weight ratio after reperfusion.
@ES The results are as follows:
@EN 1. The lungs initially flushed with Euro-Collins' solution showed marked rise in pulmonary artery pressure(PAP)(19.29±2.49 mmHg) and pulmonary vascular resistance(PVR) (55.33±12.64mmHg/ml/sec). By contrast, when the lungs were flushed with
LPD
solution initially, no rise in PAP(13.49±1.82mmHg) and PVR(15.73±4.01 mmHg/ml/sec) were observed.
2. Oxygen tension (PO2) of left atrial effluent during reperfusion was significantly higher in LPD-24 group(108.45±15.54 mmHg) and control group(124.02±6.21mmHg) than Euro-C-12 group(88.80±17.34) and Euro-C-24 group(48.53±9.70 mmHg).
Carbon dioxide tension(PCO2) during reperfusion was significantly higher in Euro-C-18 group (41.78±7.83mmHg) than with control group(30.72±0.72mmHg) and LPD-24 group(31.33±2.17mmHg).
3. PAP during reperfusion was significantly higher in Euro-C-6 group(21.17±3.43mmHg) than in control group(13.00±0.89 mmHg).
4. Wet/dry lung weight ratio after reperfusion was higher in Euro-C-12 group (6.89±0.58) than in control group(5.09±0.17) and LPD-24 group (5.36±0.40).
Lungs in LPD group showed between lung function(higher, PO2 and lower, PCO2) and lower wet/dry weight ratio than those of Euro-C group.
It is concluded that superior results in lung preservation can be achieved with pulmonary flush using extracellular fluid type solution(LPD solution) when compared with intracellular fluid type solution(Euro-Collins' solution).
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KEYWORD
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